Publications
Selected Review Articles
Saxena M, Shivdasani RA. Epigenetic Signatures and Plasticity of Intestinal and Other Stem Cells. Annu Rev Physiol. 2021; 83:405-427 | pubmed
Verzi MP, Shivdasani RA. Epigenetic regulation of intestinal stem cell differentiation. Am J Physiol Gastrointest Liver Physiol. 2020; 319(2):G189-G196 | pdf | pubmed
McCarthy N, Kraiczy J, Shivdasani RA. Cellular and molecular architecture of the intestinal stem cell niche. Nat Cell Biol. 2020; 22(9):1033-1041 | pubmed
Kim TH, Shivdasani RA. Stomach development, stem cells and disease. Development. 2016; 143(4):554-65 | pdf | pubmed
San Roman AK, Shivdasani RA. Boundaries, junctions and transitions in the gastrointestinal tract. Exp Cell Res. 2011; 317(19):2711-8 | pdf | pubmed
Mills JC, Shivdasani RA. Gastric epithelial stem cells. Gastroenterology. 2011; 140(2):412-24 | pdf | pubmed
Saxena M, Shivdasani RA. Epigenetic Signatures and Plasticity of Intestinal and Other Stem Cells. Annu Rev Physiol. 2021; 83:405-427 | pubmed
Verzi MP, Shivdasani RA. Epigenetic regulation of intestinal stem cell differentiation. Am J Physiol Gastrointest Liver Physiol. 2020; 319(2):G189-G196 | pdf | pubmed
McCarthy N, Kraiczy J, Shivdasani RA. Cellular and molecular architecture of the intestinal stem cell niche. Nat Cell Biol. 2020; 22(9):1033-1041 | pubmed
Kim TH, Shivdasani RA. Stomach development, stem cells and disease. Development. 2016; 143(4):554-65 | pdf | pubmed
San Roman AK, Shivdasani RA. Boundaries, junctions and transitions in the gastrointestinal tract. Exp Cell Res. 2011; 317(19):2711-8 | pdf | pubmed
Mills JC, Shivdasani RA. Gastric epithelial stem cells. Gastroenterology. 2011; 140(2):412-24 | pdf | pubmed
Epigenetic and Transcriptional Control Mechanisms
Gu W, Huang X, Singh PNP, Li S, Lan Y, Deng M, Lacko LA, Gomez-Salinero JM, Rafii S, Verzi M, Shivdasani RA, Zhou Q. A MTA2-SATB2 chromatin complex restrains colonic plasticity toward small intestine by retaining HNF4A at colonic chromatin. Nat Commun 2024, 15:3595. | pubmed | pdf
Singh PNP, Madha S, Leiter AB, Shivdasani RA. Cell and chromatin transitions in intestinal stem cell regeneration. Genes Dev. 2022;36:684-698. | pubmed | pdf
Singh H, Seruggia D, Madha S, Saxena M, Nagaraja AK, Wu Z, Zhou J, Huebner AJ, Maglieri A, Wezenbeek J, Hochedlinger K, Orkin SH, Bass AJ, Hornick JL, Shivdasani RA. Transcription factor-mediated intestinal metaplasia and the role of a shadow enhancer. Genes Dev. 2022;36:38-52. | pubmed | pdf
Jadhav U, Manieri E, Nalapareddy K, Madha S, Chakrabarti S, Wucherpfennig K, Barefoot M, Shivdasani RA. Replicational dilution of H3K27me3 in mammalian cells and the role of poised promoters. Mol Cell. 2020; 78:141-151 | pdf | pubmed
Jadhav U, Cavazza A, Banerjee KK, Xie H, O'Neill NK, Saenz-Vash V, Herbert Z, Madha S, Orkin SH, Zhai H, Shivdasani RA. Extensive recovery of embryonic enhancer and gene memory stored in hypomethylated enhancer DNA. Mol Cell 2019; 74:542-554 | pdf | pubmed
Banerjee KK, Saxena M, Kumar N, Chen L, Cavazza A, Toke NH, O’Neill NK, Madha S, Verzi MP, Shivdasani RA. Enhancer, transcriptional, and cell fate plasticity precedes intestinal determination during endoderm development. Genes Dev 2018; 32:1430-1442 | pdf | pubmed
Saxena M, San Roman AK, O’Neill NK, Sulahian R, Jadhav U, Shivdasani RA. Transcription factor-dependent ‘anti-repressive’ mammalian enhancers exclude H3K27me3 from extended genomic domains. Genes Dev 2017; 31:2391-2404 | pdf | pubmed
Jadhav U, Saxena M, O'Neill NK, Saadatpour A, Yuan GC, Herbert Z, Murata K, Shivdasani RA. Dynamic reorganization of chromatin accessibility signatures during dedifferentiation of secretory precursors into LGR5+ intestinal stem cells. Cell Stem Cell 2017; 21:65-77 | pdf | pubmed
Mathur R, Alver BH, San Roman AK, Wilson BG, Wang X, Agoston AT, Park PJ, Shivdasani RA, Roberts CW. ARID1A loss impairs enhancer-mediated gene regulation and drives colon cancer in mice. Nat Genet 2017 Feb; 49:296-302 | pdf | pubmed
Jadhav U, Nalapareddy K, Saxena M, O’Neill NK, Pinello L, Yuan G, Orkin SH, Shivdasani RA. Acquired tissue-specific promoter bivalency is a basis for PRC2 necessity in adult cells. Cell 2016; 165:1389-1400 | pdf | pubmed
Sarkar A, Huebner AJ, Sulahian R, Anselmo A, Xu X, Flattery K, Desai N, Sebastian C, Yram MA, Arnold K, Rivera M, Mostoslavsky R, Bronson R, Bass AJ, Sadreyev R, Shivdasani RA, Hochedlinger K. Sox2 suppresses gastric tumorigenesis in mice. Cell Rep 2016; 16:1929-1941 | pdf | pubmed
San Roman AK, Tovaglieri A, Breault DT, Shivdasani RA. Distinct processes and transcriptional targets underlie CDX2 requirements in intestinal stem cells and differentiated villus cells. Stem Cell Reports 2015; 5:673-681 | pdf | pubmed
Sulahian R, Chen J, Arany Z, Jadhav U, Peng S, Rustgi AK, Bass AJ, Srivastava A, Hornick JL, Shivdasani RA. SOX15 governs transcription in human stratified epithelia and a subset of esophageal adenocarcinomas. Cell Mol Gastroenterol Hepatol 2015; 1:598-609 | pdf | pubmed
San Roman AK, Aronson BE, Krasinski SD, Shivdasani RA, Verzi MP. Transription factors GATA4 and HNF4A control distinct aspects of intestinal crypt and villus cells in conjunction with transcription factor CDX2. J Biol Chem 2015; 290:1850-1860 | pdf | pubmed
Kim TH, Li F, Ferreiro-Neira I, Ho LL, Luyten A, Nalapareddy K, Long H, Verzi M, Shivdasani RA. Broadly permissive intestinal chromatin underlies lateral inhibition and cell plasticity. Nature 2014; 506:511-5 | pdf | pubmed
Luyten A, Zang C, Liu XS, Shivdasani RA. Active enhancers are delineated de novo during hematopoiesis, with limited lineage fidelity among specified primary blood cells. Genes Dev 2014; 28:1827-1839 | pdf | pubmed
Verzi MP, Shin H, San Roman A, Liu XS, Shivdasani RA. Intestinal master transcription factor CDX2 controls chromatin access for partner transcription factor binding. Mol Cell Biol 2013; 33:281-292 | pdf | pubmed
Ho L-L, Sinha A, Verzi M, Bernt KM, Armstrong S, Shivdasani RA. DOT1L-Mediated H3K79 methylation in chromatic is dispensible for Wnt pathway-specific and other intestinal epithelial functions. Mol Cell Biol 2013; 33:1735-1745 | pdf | pubmed
Verzi MP, Shin H, He HH, Sulahian R, Meyer CA, Montgomery RK, Fleet JC, Brown M, Liu XS, Shivdasani RA. Differentiation-specific histone modifications reveal dynamic chromatin interactions and partners for the intestinal transcription factor CDX2. Dev Cell 2010; 19:713-726 | pdf | pubmed
Verzi MP, Hatzis P, Philips J, Sulahian R, Schuijers J, Shin HG, Freed E, Brown MA, Lynch JP, Dang DT, Clevers H, Liu XS, Shivdasani RA. TCF4 and CDX2, major transcription factors for intestinal function, converge on the same cis-regulatory regions. Proc Natl Acad Sci USA 2010; 107:15151-15156. | pdf | pubmed
Gu W, Huang X, Singh PNP, Li S, Lan Y, Deng M, Lacko LA, Gomez-Salinero JM, Rafii S, Verzi M, Shivdasani RA, Zhou Q. A MTA2-SATB2 chromatin complex restrains colonic plasticity toward small intestine by retaining HNF4A at colonic chromatin. Nat Commun 2024, 15:3595. | pubmed | pdf
Singh PNP, Madha S, Leiter AB, Shivdasani RA. Cell and chromatin transitions in intestinal stem cell regeneration. Genes Dev. 2022;36:684-698. | pubmed | pdf
Singh H, Seruggia D, Madha S, Saxena M, Nagaraja AK, Wu Z, Zhou J, Huebner AJ, Maglieri A, Wezenbeek J, Hochedlinger K, Orkin SH, Bass AJ, Hornick JL, Shivdasani RA. Transcription factor-mediated intestinal metaplasia and the role of a shadow enhancer. Genes Dev. 2022;36:38-52. | pubmed | pdf
Jadhav U, Manieri E, Nalapareddy K, Madha S, Chakrabarti S, Wucherpfennig K, Barefoot M, Shivdasani RA. Replicational dilution of H3K27me3 in mammalian cells and the role of poised promoters. Mol Cell. 2020; 78:141-151 | pdf | pubmed
Jadhav U, Cavazza A, Banerjee KK, Xie H, O'Neill NK, Saenz-Vash V, Herbert Z, Madha S, Orkin SH, Zhai H, Shivdasani RA. Extensive recovery of embryonic enhancer and gene memory stored in hypomethylated enhancer DNA. Mol Cell 2019; 74:542-554 | pdf | pubmed
Banerjee KK, Saxena M, Kumar N, Chen L, Cavazza A, Toke NH, O’Neill NK, Madha S, Verzi MP, Shivdasani RA. Enhancer, transcriptional, and cell fate plasticity precedes intestinal determination during endoderm development. Genes Dev 2018; 32:1430-1442 | pdf | pubmed
Saxena M, San Roman AK, O’Neill NK, Sulahian R, Jadhav U, Shivdasani RA. Transcription factor-dependent ‘anti-repressive’ mammalian enhancers exclude H3K27me3 from extended genomic domains. Genes Dev 2017; 31:2391-2404 | pdf | pubmed
Jadhav U, Saxena M, O'Neill NK, Saadatpour A, Yuan GC, Herbert Z, Murata K, Shivdasani RA. Dynamic reorganization of chromatin accessibility signatures during dedifferentiation of secretory precursors into LGR5+ intestinal stem cells. Cell Stem Cell 2017; 21:65-77 | pdf | pubmed
Mathur R, Alver BH, San Roman AK, Wilson BG, Wang X, Agoston AT, Park PJ, Shivdasani RA, Roberts CW. ARID1A loss impairs enhancer-mediated gene regulation and drives colon cancer in mice. Nat Genet 2017 Feb; 49:296-302 | pdf | pubmed
Jadhav U, Nalapareddy K, Saxena M, O’Neill NK, Pinello L, Yuan G, Orkin SH, Shivdasani RA. Acquired tissue-specific promoter bivalency is a basis for PRC2 necessity in adult cells. Cell 2016; 165:1389-1400 | pdf | pubmed
Sarkar A, Huebner AJ, Sulahian R, Anselmo A, Xu X, Flattery K, Desai N, Sebastian C, Yram MA, Arnold K, Rivera M, Mostoslavsky R, Bronson R, Bass AJ, Sadreyev R, Shivdasani RA, Hochedlinger K. Sox2 suppresses gastric tumorigenesis in mice. Cell Rep 2016; 16:1929-1941 | pdf | pubmed
San Roman AK, Tovaglieri A, Breault DT, Shivdasani RA. Distinct processes and transcriptional targets underlie CDX2 requirements in intestinal stem cells and differentiated villus cells. Stem Cell Reports 2015; 5:673-681 | pdf | pubmed
Sulahian R, Chen J, Arany Z, Jadhav U, Peng S, Rustgi AK, Bass AJ, Srivastava A, Hornick JL, Shivdasani RA. SOX15 governs transcription in human stratified epithelia and a subset of esophageal adenocarcinomas. Cell Mol Gastroenterol Hepatol 2015; 1:598-609 | pdf | pubmed
San Roman AK, Aronson BE, Krasinski SD, Shivdasani RA, Verzi MP. Transription factors GATA4 and HNF4A control distinct aspects of intestinal crypt and villus cells in conjunction with transcription factor CDX2. J Biol Chem 2015; 290:1850-1860 | pdf | pubmed
Kim TH, Li F, Ferreiro-Neira I, Ho LL, Luyten A, Nalapareddy K, Long H, Verzi M, Shivdasani RA. Broadly permissive intestinal chromatin underlies lateral inhibition and cell plasticity. Nature 2014; 506:511-5 | pdf | pubmed
Luyten A, Zang C, Liu XS, Shivdasani RA. Active enhancers are delineated de novo during hematopoiesis, with limited lineage fidelity among specified primary blood cells. Genes Dev 2014; 28:1827-1839 | pdf | pubmed
Verzi MP, Shin H, San Roman A, Liu XS, Shivdasani RA. Intestinal master transcription factor CDX2 controls chromatin access for partner transcription factor binding. Mol Cell Biol 2013; 33:281-292 | pdf | pubmed
Ho L-L, Sinha A, Verzi M, Bernt KM, Armstrong S, Shivdasani RA. DOT1L-Mediated H3K79 methylation in chromatic is dispensible for Wnt pathway-specific and other intestinal epithelial functions. Mol Cell Biol 2013; 33:1735-1745 | pdf | pubmed
Verzi MP, Shin H, He HH, Sulahian R, Meyer CA, Montgomery RK, Fleet JC, Brown M, Liu XS, Shivdasani RA. Differentiation-specific histone modifications reveal dynamic chromatin interactions and partners for the intestinal transcription factor CDX2. Dev Cell 2010; 19:713-726 | pdf | pubmed
Verzi MP, Hatzis P, Philips J, Sulahian R, Schuijers J, Shin HG, Freed E, Brown MA, Lynch JP, Dang DT, Clevers H, Liu XS, Shivdasani RA. TCF4 and CDX2, major transcription factors for intestinal function, converge on the same cis-regulatory regions. Proc Natl Acad Sci USA 2010; 107:15151-15156. | pdf | pubmed
Gastrointestinal Differentiation, Development, and Disease
Singh PNP, Gu W, Madha S, Lynch AW, Cejas P, He R, Bhattacharya S, Muñoz Gomez M, Oser MG, Brown M, Long HW, Meyer CA, Zhou Q, Shivdasani RA. Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation. Cell Stem Cell 2024, 31:1038-1057. | pubmed
Manieri E, Tie G, Malagola E, Seruggia D, Madha S, Maglieri A, Huang K, Fujiwara Y, Zhang K, Orkin SH, Wang TC, He R, McCarthy N, Shivdasani RA. Role of PDGFRA+ cells and a CD55+ PDGFRALo fraction in the gastric mesenchymal niche. Nat Commun 2023, 14:7978. | pubmed | pdf
Kraiczy J, McCarthy N, Malagola E, Tie G, Madha S, Boffelli D, Wagner D, Wang TC, Shivdasani RA. Graded BMP signaling within intestinal crypt architecture directs self-organization of the Wnt-secreting stem cell niche. Cell Stem Cell 2023, 30:433-449. | pubmed | pdf
McCarthy N, Tie G, Madha S, Maglieri A, Kraiczy J, Shivdasani RA. Smooth muscle contributes to the development and function of a layered intestinal stem cell niche. Dev Cell 2023, 58:550-564. | pubmed | pdf
Gu W, Wang H, Huang X, Kraiczy J, Singh PNP, Ng C, Dagdeviren S, Houghton S, Pellon-Cardenas O, Lan Y, Nie Y, Zhang J, Banerjee KK, Onufer EJ, Warner BW, Spence J, Scherl E, Rafii S, Lee RT, Verzi MP, Redmond D, Longman R, Helin K, Shivdasani RA, Zhou Q. SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling. Cell Stem Cell. 2022;29:101-115.| pubmed | pdf
Singh H, Ha K, Hornick JL, Madha S, Cejas P, Jajoo K, Singh P, Polak P, Lee H, Shivdasani RA. Hybrid stomach-intestinal chromatin states underlie human Barrett's metaplasia. Gastroenterology. 2021;161:924-939. | pdf | pubmed
Rao-Bhatia A, Zhu M, Yin WC, Coquenlorge S, Zhang X, Woo J, Sun Y, Dean CH, Liu A, Hui CC, Shivdasani RA, McNeill H, Hopyan S, Kim TH. Hedgehog-activated Fat4 and PCP pathways mediate mesenchymal cell clustering and villus formation in gut development. Dev Cell. 2020; 52:647-658. | pdf | pubmed
Murata K, Jadhav U, Madha S, van Es J, Dean J, Cavazza A, Wucherpfennig K, Michor F, Clevers H, Shivdasani RA. Ascl2-dependent cell dedifferentiation drives regeneration of ablated intestinal stem cells. Cell Stem Cell. 2020; 26:377-390. | pdf | pubmed
McCarthy N, Manieri E, Storm EE, Saadatpour A, Luoma AM, Kapoor VN, Madha S, Gaynor LT, Cox C, Keerthivasan S, Wucherpfennig K, Yuan GC, de Sauvage FJ, Turley SJ, Shivdasani RA. Distinct mesenchymal cell populations generate the essential intestinal BMP signaling gradient. Cell Stem Cell. 2020; 26:391-402. | pdf | pubmed
Kim CK, Saxena M, Maharjan K, Song JJ, Shroyer KR, Bialkowska AB, Shivdasani RA, Yang VW. Krüppel-like Factor 5 regulates stemness, lineage specification, and regeneration of intestinal epithelial stem cells. Cell Mol Gastroenterol Hepatol. 2020;9(4):587-609 | pdf | pubmed
Banerjee KK, Saxena M, Kumar N, Chen L, Cavazza A, Toke NH, O’Neill NK, Madha S, Verzi MP, Shivdasani RA. Enhancer, transcriptional, and cell fate plasticity precedes intestinal determination during endoderm development. Genes Dev 2018; 32:1430-1442 | pdf | pubmed
Jadhav U, Saxena M, O'Neill NK, Saadatpour A, Yuan GC, Herbert Z, Murata K, Shivdasani RA. Dynamic reorganization of chromatin accessibility signatures during dedifferentiation of secretory precursors into LGR5+ intestinal stem cells. Cell Stem Cell 2017; 21:65-77 | pdf | pubmed
Jadhav U, Nalapareddy K, Saxena M, O’Neill NK, Pinello L, Yuan G, Orkin SH, Shivdasani RA. Acquired tissue-specific promoter bivalency is a basis for PRC2 necessity in adult cells. Cell 2016; 165:1389-1400 | pdf | pubmed
Kim TH, Saadatpour A, Guo G, Saxena M, Cavazza A, Desai N, Jadhav U, Jiang L, Rivera MN, Orkin SH, Yuan GC, Shivdasani RA. Single-cell transcript profiles reveal multilineage priming in early progenitors derived from LGR5+ intestinal stem cells. Cell Rep 2016; 16:2053-2060 | pdf | pubmed
Sarkar A, Huebner AJ, Sulahian R, Anselmo A, Xu X, Flattery K, Desai N, Sebastian C, Yram MA, Arnold K, Rivera M, Mostoslavsky R, Bronson R, Bass AJ, Sadreyev R, Shivdasani RA, Hochedlinger K. Sox2 suppresses gastric tumorigenesis in mice. Cell Rep 2016; 16:1929-1941 | pdf | pubmed
San Roman AK, Tovaglieri A, Breault DT, Shivdasani RA. Distinct processes and transciptional targets underlie CDX2 requirements in intestinal stem cells and differentiated villus cells. Stem Cell Reports 2015; 5:673-681 | pdf | pubmed
Sulahian R, Chen J, Arany Z, Jadhav U, Peng S, Rustgi AK, Bass AJ, Srivastava A, Hornick JL, Shivdasani RA. SOX15 governs transription in human stratified epithelia and a subset of esophageal adenocarcinomas. Cell Mol Gastroenterol Hepatol 2015; 1:598-609 | pdf | pubmed
Jayewickreme C, Shivdasani RA. Control of stomach smooth muscle development and intestinal rotation by transcription factor BARX1. Dev Biol 2015; 405:21-32 | pdf | pubmed
San Roman AK, Aronson BE, Krasinski SD, Shivdasani RA, Verzi MP. Transription factors GATA4 and HNF4A control distinct aspects of intestinal crypt and villus cells in conjunction with transcription factor CDX2. J Biol Chem 2015; 290:1850-1860 | pdf | pubmed
Morris SA, Cahan P, Li H, Zhao AM, San Roman AK, Shivdasani RA, Collins JJ, Daley GQ. Dissecting engineered cell types and enhancing cell fate conversion via CellNet. Cell 2014; 158:889-902 | pdf | pubmed
Feng R, Aihara E, Kenny S, Yang L, Li J, Varro A, Montrose MH, Shroyer NF, Wang TC, Shivdasani RA, Zavros Y. Indian hedgehog mediates gastrin-induced proliferation in stomach of adult mice. Gastroenterology 2014; | pdf | pubmed
San Roman AK, Jayewickreme CD, Murtaugh LC, Shivdasani RA. Wnt secretion from epithelial cells and subepithelial myofibroblasts is not required in the mouse intestinal stem cell niche in vivo. Stem Cell Reports 2014; 2:127-34 | pdf | pubmed
Kim TH, Li F, Ferreiro-Neira I, Ho LL, Luyten A, Nalapareddy K, Long H, Verzi M, Shivdasani RA. Broadly permissive intestinal chromatin underlies lateral inhibition and cell plasticity. Nature 2014; 506:511-5 | pdf | pubmed
Verzi MP, Shin H, San Roman A, Liu XS, Shivdasani RA. Intestinal master transcription factor CDX2 controls chromatin access for partner transcription factor binding. Mol Cell Biol 2013; 33:281-292 | pdf | pubmed
Pignon J-C, Grisanzio C, Yan G, Song J, Shivdasani RA, Signoretti S. p63-expressing cells are the stem cells of developing prostate, bladder, and colorectal epithelia. PNAS USA 2013; 110:8105-8110 | pdf | pubmed
Ho L-L, Sinha A, Verzi M, Bernt KM, Armstrong S, Shivdasani RA. DOT1L-Mediated H3K79 methylation in chromatic is dispensible for Wnt pathway-specific and other intestinal epithelial functions. Mol Cell Biol 2013; 33:1735-1745 | pdf | pubmed
Kim T-H, Escudero S, Shivdasani RA. Intact function of Lgr5 receptor-expressing intestinal stem cells in the absence of Paneth cells. Proc Natl Acad Sci USA 2012; 109:3932-3937 | pdf | pubmed
Woo J, Miletich I, Kim B-M, Sharpe PT, Shivdasani RA. Barx1-mediated inhibition of Wnt signalling in the mouse thoracic foregut controls tracheo-esophageal septation and epithelial differentiation. PLoS One 2011; 6:e22493 | pdf | pubmed
Kim T-H, Kim B-M, Mao J, Rowan S, Shivdasani RA. Endodermal Hedgehog signals modulate Notch pathway activity in the developing digestive tract mesenchyme. Development 2011; 138:3225-3233 | pdf |pubmed
Verzi MP, Shin H, Ho L, Liu XS, Shivdasani RA. Essential and Redundant Functions of Caudal Family Proteins in Activating Adult Intestinal Genes. Mol Cell Biol 2011; 31:2026-2039 | pdf | pubmed
Kim T-H, Shivdasani RA. Notch signaling in stomach epithelial stem cell homeostasis. J Exp Med 2011; 208(4):677-688 | pdf | pubmed
Kim T-H, Shivdasani RA. Genetic Evidence That Intestinal Notch Functions Vary Regionally and Operate through a Common Mechanism of Math1 Repression. J Biol Chem 2011; 286(13):11427-11433 | pdf | pubmed
Kim B-M, Woo J, Kanellopoulou C, Shivdasani RA. Regulation of mouse stomach development and Barx1 expression by specific microRNAs. Development 2011; 138:1081-1086 | pdf | pubmed
Verzi MP, Shin H, He HH, Sulahian R, Meyer CA, Montgomery RK, Fleet JC, Brown M, Liu XS, Shivdasani RA. Differentiation-specific histone modifications reveal dynamic chromatin interactions and partners for the intestinal transcription factor CDX2. Dev Cell 2010; 19:713-726 | pdf | pubmed
Horst D, Gu X, Bhasin M, Yang Q, Verzi M, Lin D, Joseph M, Zhang X, Chen W, Li Y-P, Shivdasani RA, Libermann TA. Requirement of the epithelium-specific Ets transcription factor Spdef for mucous gland cell function in the gastric antrum. J Biol Chem 2010; 285(45):35047-55 | pdf | pubmed
Verzi MP, Hatzis P, Philips J, Sulahian R, Schuijers J, Shin HG, Freed E, Brown MA, Lynch JP, Dang DT, Clevers H, Liu XS, Shivdasani RA. TCF4 and CDX2, major transcription factors for intestinal function, converge on the same cis-regulatory regions. Proc Natl Acad Sci USA 2010; 107:15151-15156.
doi: 10.1073/pnas.1003822107 | pdf | pubmed
Mao J, Kim B-M, Rajurkar M, Shivdasani RA, McMahon AP. Hedgehog signaling controls mesenchymal growth in the developing mammalian digestive tract. Development 2010; 137:1721-1729 | pdf | pubmed
Verzi MP, Stanfel MN, Moses KA, Kim B-M, Zhang Y, Schwartz RJ, Shivdasani RA, Zimmer WE. Role of the homeodomain transcription factor Bapx1 in mouse distal stomach development. Gastroenterology 2009; 136:1701-1710 | pdf | pubmed
Verzi MP, Khan AH, Ito S, Shivdasani RA. The forkhead transcription factor Foxq1 controls mucin gene expression and secretory granule biogenesis in mouse stomach surface mucous cells. Gastroenterology 2008; 135:591-600 | pdf | pubmed
Choi MY, Romer AI, Wang Y, Wu MP, Ito S, Leiter AB, Shivdasani RA. Requirement of the tissue-restricted homeodomain transcription factor Nkx6.3 in differentiation of gastrin-producing G-cells in the stomach antrum. Mol Cell Biol 2008; 28:3208-3218 | pdf | pubmed
Van Dyck F, Braem CV, Chen Z, Declercq J, Deckers R, Kim B-M, Ito S, Wu MK, Cohen DE, Dewerchin M, Derua R, Waelkens E, Fiette L, Roebroek A, Schuit F, Van de Ven WJM, Shivdasani RA. Loss of the PlagL2 transcription factor affects lacteal uptake of chylomicrons. Cell Metabolism 2007; 6:406-413 | pdf | pubmed
Kim B-M, Miletich I, Mao J, McMahon AP, Sharpe PA, Shivdasani RA. Independent functions and mechanisms for homeobox gene Barx1 in patterning mouse stomach and spleen. Development 2007; 134:3603-3613 | pdf | pubmed
Kim B-M, Mao J, Taketo MM, Shivdasani RA. Phases of canonical Wnt signaling during the development of mouse intestinal epithelium. Gastroenterology 2007; 133:529-538 | pdf | pubmed
Choi MY, Romer AI, Hu M, Lepourcelet M, Mechoor A, Yesilaltay A, Krieger M, Gray PA, Shivdasani RA. A dynamic expression survey identifies transcription factors relevant in mouse digestive tract development. Development 2006; 133(20):4119-29 | pdf | pubmed
Hu M, Shivdasani RA. Overlapping gene expression in fetal mouse intestine development and human colorectal cancers. Cancer Research 2005; 65:8715-8722 | pdf | pubmed
Kim BM, Buchner G, Miletich I, Sharpe PT, Shivdasani RA. The stomach mesenchymal transcription factor Barx1 specifies gastric epithelial identity through inhibition of transient Wnt signaling. Dev Cell 2005; 8:611-622 | pdf | pubmed
Drori S, Girnun GD, Tou L, Szwaya JD, Mueller E, Kia X, Shivdasani RA, Spiegelman B Hic-5 regulates an epithelial program mediated by PPAR-y. Genes & Development 2005; 19:362-375 | pdf | pubmed
Lepourcelet M, Tou L, Cai L, Sawada J, Lazar AF, Glickman JN, Williamson J, Everett AD, Redston M, Fox EA, Nakatani Y, Shivdasani RA. Insights into developmental mechanisms and cancers in the mammalian intestine derived from SAGE and study of the hepatoma-derived growth factor (HDGF).
Development 2005; 132:415-427 | pdf | pubmed
Tou L, Liu Q, Shivdasani RA. Regulation of mammalian epithelial differentiation and intestine development by class I histone deacetylases. Mol Cell Biol 2004; 24:3132-3139 | pdf | pubmed
Malik TH, von Stechow D, Bronson RT, Shivdasani RA. Deletion of the GATA domain of TRPS1 causes an absence of facial hair and provides new insights into bone disorder in inherited tricho-rhino-phalangeal syndromes. Mol Cell Biol 2002; 22:8592-8600 | pdf | pubmed
Shivdasani, RA. Molecular Regulation of Vertebrate Early Endoderm Development. Dev Biol 2002; 249:191-203 [Review] | pdf | pubmed
Lepourcelet M, Shivdasani RA. Characterization of a novel mammalian Groucho isoform and its role in transcriptional regulation. J Biol Chem 2002; 49:47732-47740 | pdf | pubmed
Zhang J, Rosenthal A, de Sauvage FJ, Shivdasani RA. Downregulation of hedgehog signaling is required NF-E2, FLI1 and RUNX1 collaborate at areas of dynamic chromatin to activate transcription in mature mouse megakaryocytes for organogenesis of the small intestine in Xenopus. Dev Biol 2001; 229:188-202 | pdf | pubmed
Malik TH, Shoichet SA, Latham P, Kroll T, Peters LL, Shivdasani RA. Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1. EMBO J 2001; 20:1715-1725 | pdf | pubmed
Shoichet SA, Malik TH, Rothman JH, Shivdasani RA. Action of the C. elegans GATA factor END-1 in Xenopus suggests that similar mechanisms initiate endoderm development in ecdysozoa and vertebrates. Proc Natl Acad Sci USA 2000; 97:4076-4081 | pdf | pubmed
Malik TH, Shivdasani RA. Structure and expression of a novel Frizzled gene isolated from the developing mouse gut. Biochem J 2000; 349:829-834 | pdf | pubmed
Lee YJ, Swencki B, Shoichet S, Shivdasani RA. A possible role for the High Mobility Group-box transcription factor Tcf-4 in vertebrate gut epithelial cell differentiation. J Biol Chem 1999; 274:1566-1572 | pdf | pubmed
Singh PNP, Gu W, Madha S, Lynch AW, Cejas P, He R, Bhattacharya S, Muñoz Gomez M, Oser MG, Brown M, Long HW, Meyer CA, Zhou Q, Shivdasani RA. Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation. Cell Stem Cell 2024, 31:1038-1057. | pubmed
Manieri E, Tie G, Malagola E, Seruggia D, Madha S, Maglieri A, Huang K, Fujiwara Y, Zhang K, Orkin SH, Wang TC, He R, McCarthy N, Shivdasani RA. Role of PDGFRA+ cells and a CD55+ PDGFRALo fraction in the gastric mesenchymal niche. Nat Commun 2023, 14:7978. | pubmed | pdf
Kraiczy J, McCarthy N, Malagola E, Tie G, Madha S, Boffelli D, Wagner D, Wang TC, Shivdasani RA. Graded BMP signaling within intestinal crypt architecture directs self-organization of the Wnt-secreting stem cell niche. Cell Stem Cell 2023, 30:433-449. | pubmed | pdf
McCarthy N, Tie G, Madha S, Maglieri A, Kraiczy J, Shivdasani RA. Smooth muscle contributes to the development and function of a layered intestinal stem cell niche. Dev Cell 2023, 58:550-564. | pubmed | pdf
Gu W, Wang H, Huang X, Kraiczy J, Singh PNP, Ng C, Dagdeviren S, Houghton S, Pellon-Cardenas O, Lan Y, Nie Y, Zhang J, Banerjee KK, Onufer EJ, Warner BW, Spence J, Scherl E, Rafii S, Lee RT, Verzi MP, Redmond D, Longman R, Helin K, Shivdasani RA, Zhou Q. SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling. Cell Stem Cell. 2022;29:101-115.| pubmed | pdf
Singh H, Ha K, Hornick JL, Madha S, Cejas P, Jajoo K, Singh P, Polak P, Lee H, Shivdasani RA. Hybrid stomach-intestinal chromatin states underlie human Barrett's metaplasia. Gastroenterology. 2021;161:924-939. | pdf | pubmed
Rao-Bhatia A, Zhu M, Yin WC, Coquenlorge S, Zhang X, Woo J, Sun Y, Dean CH, Liu A, Hui CC, Shivdasani RA, McNeill H, Hopyan S, Kim TH. Hedgehog-activated Fat4 and PCP pathways mediate mesenchymal cell clustering and villus formation in gut development. Dev Cell. 2020; 52:647-658. | pdf | pubmed
Murata K, Jadhav U, Madha S, van Es J, Dean J, Cavazza A, Wucherpfennig K, Michor F, Clevers H, Shivdasani RA. Ascl2-dependent cell dedifferentiation drives regeneration of ablated intestinal stem cells. Cell Stem Cell. 2020; 26:377-390. | pdf | pubmed
McCarthy N, Manieri E, Storm EE, Saadatpour A, Luoma AM, Kapoor VN, Madha S, Gaynor LT, Cox C, Keerthivasan S, Wucherpfennig K, Yuan GC, de Sauvage FJ, Turley SJ, Shivdasani RA. Distinct mesenchymal cell populations generate the essential intestinal BMP signaling gradient. Cell Stem Cell. 2020; 26:391-402. | pdf | pubmed
Kim CK, Saxena M, Maharjan K, Song JJ, Shroyer KR, Bialkowska AB, Shivdasani RA, Yang VW. Krüppel-like Factor 5 regulates stemness, lineage specification, and regeneration of intestinal epithelial stem cells. Cell Mol Gastroenterol Hepatol. 2020;9(4):587-609 | pdf | pubmed
Banerjee KK, Saxena M, Kumar N, Chen L, Cavazza A, Toke NH, O’Neill NK, Madha S, Verzi MP, Shivdasani RA. Enhancer, transcriptional, and cell fate plasticity precedes intestinal determination during endoderm development. Genes Dev 2018; 32:1430-1442 | pdf | pubmed
Jadhav U, Saxena M, O'Neill NK, Saadatpour A, Yuan GC, Herbert Z, Murata K, Shivdasani RA. Dynamic reorganization of chromatin accessibility signatures during dedifferentiation of secretory precursors into LGR5+ intestinal stem cells. Cell Stem Cell 2017; 21:65-77 | pdf | pubmed
Jadhav U, Nalapareddy K, Saxena M, O’Neill NK, Pinello L, Yuan G, Orkin SH, Shivdasani RA. Acquired tissue-specific promoter bivalency is a basis for PRC2 necessity in adult cells. Cell 2016; 165:1389-1400 | pdf | pubmed
Kim TH, Saadatpour A, Guo G, Saxena M, Cavazza A, Desai N, Jadhav U, Jiang L, Rivera MN, Orkin SH, Yuan GC, Shivdasani RA. Single-cell transcript profiles reveal multilineage priming in early progenitors derived from LGR5+ intestinal stem cells. Cell Rep 2016; 16:2053-2060 | pdf | pubmed
Sarkar A, Huebner AJ, Sulahian R, Anselmo A, Xu X, Flattery K, Desai N, Sebastian C, Yram MA, Arnold K, Rivera M, Mostoslavsky R, Bronson R, Bass AJ, Sadreyev R, Shivdasani RA, Hochedlinger K. Sox2 suppresses gastric tumorigenesis in mice. Cell Rep 2016; 16:1929-1941 | pdf | pubmed
San Roman AK, Tovaglieri A, Breault DT, Shivdasani RA. Distinct processes and transciptional targets underlie CDX2 requirements in intestinal stem cells and differentiated villus cells. Stem Cell Reports 2015; 5:673-681 | pdf | pubmed
Sulahian R, Chen J, Arany Z, Jadhav U, Peng S, Rustgi AK, Bass AJ, Srivastava A, Hornick JL, Shivdasani RA. SOX15 governs transription in human stratified epithelia and a subset of esophageal adenocarcinomas. Cell Mol Gastroenterol Hepatol 2015; 1:598-609 | pdf | pubmed
Jayewickreme C, Shivdasani RA. Control of stomach smooth muscle development and intestinal rotation by transcription factor BARX1. Dev Biol 2015; 405:21-32 | pdf | pubmed
San Roman AK, Aronson BE, Krasinski SD, Shivdasani RA, Verzi MP. Transription factors GATA4 and HNF4A control distinct aspects of intestinal crypt and villus cells in conjunction with transcription factor CDX2. J Biol Chem 2015; 290:1850-1860 | pdf | pubmed
Morris SA, Cahan P, Li H, Zhao AM, San Roman AK, Shivdasani RA, Collins JJ, Daley GQ. Dissecting engineered cell types and enhancing cell fate conversion via CellNet. Cell 2014; 158:889-902 | pdf | pubmed
Feng R, Aihara E, Kenny S, Yang L, Li J, Varro A, Montrose MH, Shroyer NF, Wang TC, Shivdasani RA, Zavros Y. Indian hedgehog mediates gastrin-induced proliferation in stomach of adult mice. Gastroenterology 2014; | pdf | pubmed
San Roman AK, Jayewickreme CD, Murtaugh LC, Shivdasani RA. Wnt secretion from epithelial cells and subepithelial myofibroblasts is not required in the mouse intestinal stem cell niche in vivo. Stem Cell Reports 2014; 2:127-34 | pdf | pubmed
Kim TH, Li F, Ferreiro-Neira I, Ho LL, Luyten A, Nalapareddy K, Long H, Verzi M, Shivdasani RA. Broadly permissive intestinal chromatin underlies lateral inhibition and cell plasticity. Nature 2014; 506:511-5 | pdf | pubmed
Verzi MP, Shin H, San Roman A, Liu XS, Shivdasani RA. Intestinal master transcription factor CDX2 controls chromatin access for partner transcription factor binding. Mol Cell Biol 2013; 33:281-292 | pdf | pubmed
Pignon J-C, Grisanzio C, Yan G, Song J, Shivdasani RA, Signoretti S. p63-expressing cells are the stem cells of developing prostate, bladder, and colorectal epithelia. PNAS USA 2013; 110:8105-8110 | pdf | pubmed
Ho L-L, Sinha A, Verzi M, Bernt KM, Armstrong S, Shivdasani RA. DOT1L-Mediated H3K79 methylation in chromatic is dispensible for Wnt pathway-specific and other intestinal epithelial functions. Mol Cell Biol 2013; 33:1735-1745 | pdf | pubmed
Kim T-H, Escudero S, Shivdasani RA. Intact function of Lgr5 receptor-expressing intestinal stem cells in the absence of Paneth cells. Proc Natl Acad Sci USA 2012; 109:3932-3937 | pdf | pubmed
Woo J, Miletich I, Kim B-M, Sharpe PT, Shivdasani RA. Barx1-mediated inhibition of Wnt signalling in the mouse thoracic foregut controls tracheo-esophageal septation and epithelial differentiation. PLoS One 2011; 6:e22493 | pdf | pubmed
Kim T-H, Kim B-M, Mao J, Rowan S, Shivdasani RA. Endodermal Hedgehog signals modulate Notch pathway activity in the developing digestive tract mesenchyme. Development 2011; 138:3225-3233 | pdf |pubmed
Verzi MP, Shin H, Ho L, Liu XS, Shivdasani RA. Essential and Redundant Functions of Caudal Family Proteins in Activating Adult Intestinal Genes. Mol Cell Biol 2011; 31:2026-2039 | pdf | pubmed
Kim T-H, Shivdasani RA. Notch signaling in stomach epithelial stem cell homeostasis. J Exp Med 2011; 208(4):677-688 | pdf | pubmed
Kim T-H, Shivdasani RA. Genetic Evidence That Intestinal Notch Functions Vary Regionally and Operate through a Common Mechanism of Math1 Repression. J Biol Chem 2011; 286(13):11427-11433 | pdf | pubmed
Kim B-M, Woo J, Kanellopoulou C, Shivdasani RA. Regulation of mouse stomach development and Barx1 expression by specific microRNAs. Development 2011; 138:1081-1086 | pdf | pubmed
Verzi MP, Shin H, He HH, Sulahian R, Meyer CA, Montgomery RK, Fleet JC, Brown M, Liu XS, Shivdasani RA. Differentiation-specific histone modifications reveal dynamic chromatin interactions and partners for the intestinal transcription factor CDX2. Dev Cell 2010; 19:713-726 | pdf | pubmed
Horst D, Gu X, Bhasin M, Yang Q, Verzi M, Lin D, Joseph M, Zhang X, Chen W, Li Y-P, Shivdasani RA, Libermann TA. Requirement of the epithelium-specific Ets transcription factor Spdef for mucous gland cell function in the gastric antrum. J Biol Chem 2010; 285(45):35047-55 | pdf | pubmed
Verzi MP, Hatzis P, Philips J, Sulahian R, Schuijers J, Shin HG, Freed E, Brown MA, Lynch JP, Dang DT, Clevers H, Liu XS, Shivdasani RA. TCF4 and CDX2, major transcription factors for intestinal function, converge on the same cis-regulatory regions. Proc Natl Acad Sci USA 2010; 107:15151-15156.
doi: 10.1073/pnas.1003822107 | pdf | pubmed
Mao J, Kim B-M, Rajurkar M, Shivdasani RA, McMahon AP. Hedgehog signaling controls mesenchymal growth in the developing mammalian digestive tract. Development 2010; 137:1721-1729 | pdf | pubmed
Verzi MP, Stanfel MN, Moses KA, Kim B-M, Zhang Y, Schwartz RJ, Shivdasani RA, Zimmer WE. Role of the homeodomain transcription factor Bapx1 in mouse distal stomach development. Gastroenterology 2009; 136:1701-1710 | pdf | pubmed
Verzi MP, Khan AH, Ito S, Shivdasani RA. The forkhead transcription factor Foxq1 controls mucin gene expression and secretory granule biogenesis in mouse stomach surface mucous cells. Gastroenterology 2008; 135:591-600 | pdf | pubmed
Choi MY, Romer AI, Wang Y, Wu MP, Ito S, Leiter AB, Shivdasani RA. Requirement of the tissue-restricted homeodomain transcription factor Nkx6.3 in differentiation of gastrin-producing G-cells in the stomach antrum. Mol Cell Biol 2008; 28:3208-3218 | pdf | pubmed
Van Dyck F, Braem CV, Chen Z, Declercq J, Deckers R, Kim B-M, Ito S, Wu MK, Cohen DE, Dewerchin M, Derua R, Waelkens E, Fiette L, Roebroek A, Schuit F, Van de Ven WJM, Shivdasani RA. Loss of the PlagL2 transcription factor affects lacteal uptake of chylomicrons. Cell Metabolism 2007; 6:406-413 | pdf | pubmed
Kim B-M, Miletich I, Mao J, McMahon AP, Sharpe PA, Shivdasani RA. Independent functions and mechanisms for homeobox gene Barx1 in patterning mouse stomach and spleen. Development 2007; 134:3603-3613 | pdf | pubmed
Kim B-M, Mao J, Taketo MM, Shivdasani RA. Phases of canonical Wnt signaling during the development of mouse intestinal epithelium. Gastroenterology 2007; 133:529-538 | pdf | pubmed
Choi MY, Romer AI, Hu M, Lepourcelet M, Mechoor A, Yesilaltay A, Krieger M, Gray PA, Shivdasani RA. A dynamic expression survey identifies transcription factors relevant in mouse digestive tract development. Development 2006; 133(20):4119-29 | pdf | pubmed
Hu M, Shivdasani RA. Overlapping gene expression in fetal mouse intestine development and human colorectal cancers. Cancer Research 2005; 65:8715-8722 | pdf | pubmed
Kim BM, Buchner G, Miletich I, Sharpe PT, Shivdasani RA. The stomach mesenchymal transcription factor Barx1 specifies gastric epithelial identity through inhibition of transient Wnt signaling. Dev Cell 2005; 8:611-622 | pdf | pubmed
Drori S, Girnun GD, Tou L, Szwaya JD, Mueller E, Kia X, Shivdasani RA, Spiegelman B Hic-5 regulates an epithelial program mediated by PPAR-y. Genes & Development 2005; 19:362-375 | pdf | pubmed
Lepourcelet M, Tou L, Cai L, Sawada J, Lazar AF, Glickman JN, Williamson J, Everett AD, Redston M, Fox EA, Nakatani Y, Shivdasani RA. Insights into developmental mechanisms and cancers in the mammalian intestine derived from SAGE and study of the hepatoma-derived growth factor (HDGF).
Development 2005; 132:415-427 | pdf | pubmed
Tou L, Liu Q, Shivdasani RA. Regulation of mammalian epithelial differentiation and intestine development by class I histone deacetylases. Mol Cell Biol 2004; 24:3132-3139 | pdf | pubmed
Malik TH, von Stechow D, Bronson RT, Shivdasani RA. Deletion of the GATA domain of TRPS1 causes an absence of facial hair and provides new insights into bone disorder in inherited tricho-rhino-phalangeal syndromes. Mol Cell Biol 2002; 22:8592-8600 | pdf | pubmed
Shivdasani, RA. Molecular Regulation of Vertebrate Early Endoderm Development. Dev Biol 2002; 249:191-203 [Review] | pdf | pubmed
Lepourcelet M, Shivdasani RA. Characterization of a novel mammalian Groucho isoform and its role in transcriptional regulation. J Biol Chem 2002; 49:47732-47740 | pdf | pubmed
Zhang J, Rosenthal A, de Sauvage FJ, Shivdasani RA. Downregulation of hedgehog signaling is required NF-E2, FLI1 and RUNX1 collaborate at areas of dynamic chromatin to activate transcription in mature mouse megakaryocytes for organogenesis of the small intestine in Xenopus. Dev Biol 2001; 229:188-202 | pdf | pubmed
Malik TH, Shoichet SA, Latham P, Kroll T, Peters LL, Shivdasani RA. Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1. EMBO J 2001; 20:1715-1725 | pdf | pubmed
Shoichet SA, Malik TH, Rothman JH, Shivdasani RA. Action of the C. elegans GATA factor END-1 in Xenopus suggests that similar mechanisms initiate endoderm development in ecdysozoa and vertebrates. Proc Natl Acad Sci USA 2000; 97:4076-4081 | pdf | pubmed
Malik TH, Shivdasani RA. Structure and expression of a novel Frizzled gene isolated from the developing mouse gut. Biochem J 2000; 349:829-834 | pdf | pubmed
Lee YJ, Swencki B, Shoichet S, Shivdasani RA. A possible role for the High Mobility Group-box transcription factor Tcf-4 in vertebrate gut epithelial cell differentiation. J Biol Chem 1999; 274:1566-1572 | pdf | pubmed
Cancer Biology and Therapeutics
Cejas P, Drier Y, Dreijerink KMA, Brosens LAA, Deshpande V, Epstein CB, Conemans EB, Morsink FHM, Graham MK, Valk GD, Vriens MR, Castillo CF, Ferrone CR, Adar T, Bowden M, Whitton HJ, Da Silva A, Font-Tello A, Long HW, Gaskell E, Shoresh N, Heaphy CM, Sicinska E, Kulke MH, Chung DC, Bernstein BE, Shivdasani RA. Enhancer signatures stratify and predict outcomes of non-functional pancreatic neuroendocrine tumors. Nat Med 2019; 25:1260-1265 | pdf | pubmed
Witwicki RM, Ekram MB, Qiu X, Janiszewska M, Shu S, Kwon M, Trinh A, Frias E, Ramadan N, Hoffman G, Yu K, Xie Y, McAllister G, McDonald R, Golji J, Schlabach M, deWeck A, Keen N, Chan HM, Ruddy D, Rejtar T, Sovath S, Silver S, Sellers WR, Jagani Z, Hogarty MD, Roberts CMW, Brown M, Stegmaier K, Long H, Shivdasani RA, Pellman D, Polyak K. TRPS1 is a lineage-specific transcriptional dependency in breast cancer. Cell Rep 2018; 25:1255-1267.e5 | pdf | pubmed
Schumacher SE, Shim BY, Corso G, Ryu MH, Kang YK, Roviello F, Saksena G, Peng S, Shivdasani RA, Bass AJ, Beroukhim R. Somatic copy number alterations in gastric adenocarcinomas among Asian and Western patients. PLoS One. 2017;12(4):e0176045 | pdf | pubmed
Cejas P, Cavazza A, Yandava CN, Moreno V, Horst D, Moreno-Rubio J, Burgos E, Mendiola M, Taing L, Goel A, Feliu J, Shivdasani RA. Transcriptional regulator CNOT3 defines an aggressive colorectal cancer subtype. Cancer Res 2017; 77:766-779 | pdf | pubmed
Cejas P, Li L, O'Neill NK, Duarte M, Rao P, Bowden M, Zhou CW, Mendiola M, Burgos E, Feliu J, Moreno-Rubio J, Guadalajara H, Moreno V, García-Olmo D, Bellmunt J, Mullane S, Hirsch M, Sweeney CJ, Richardson A, Liu XS, Brown M, Shivdasani RA, Long HW. Chromatin immunoprecipitation from fixed clinical tissues reveals tumor-specific enhancer profiles. Nat Med 2016; 22:685-691 | pdf | pubmed
Pomerantz MM, Li F, Takeda DY, Lenci R, Chonkar A, Chabot M, Cejas P, Vazquez F, Cook J, Shivdasani RA, Bowden M, Lis R, Hahn WC1,4, Kantoff PW1, Brown M, Loda M, Long HW, Freedman ML. The androgen receptor cistrome is extensively reprogrammed in human prostate tumorgenesis. Nat Genet 2015; 47:1346-1351 | pdf | pubmed
Sulahian R, Casey F, Shen J, Qian ZR, Shin H, Ogino S, Weir BA, Vazquez F, Liu XS, Hahn WC, Bass AJ, Chan V, Shivdasani RA. An integrative analysis reveals functional targets of GATA6 transcriptional regulation in gastric cancer. Oncogene 2014; 33:5637-5648 | pdf | pubmed
Francis J, Kiezun A, Ramos AH, Serra S, Pedamallu CS, Qian ZR, Banck MS, Kanwar R, Kulkarni AA, Karpathikis A, Manzo V, Contractor T, Philips J, Nickerson R, Pho N, Hooshmand SM, Brais LK, Lawrence M, Pugh T, McKenna A, Sivachenko A, Cibulskis K, Carter SL, Ojesina AI, Freeman S, Jones RT, Voet D, Saksena G, Auclair D, Onofrio R, Shefler E, Sougnez C, Grimsby J, Green L, Lennon N, Meyer T, Caplin M, Chung DC, Beutler AS, Ogino S, Thirlwell C, Shivdasani RA, Asa SL, Harris CR, Getz G, Kulke MH, Meyerson M. Somatic mutation of CDKN1B in small intestine neuroendocrine tumors. Nat Genet 2013; 45:1483-1486 | pdf | pubmed
Horst D, Chen J, Morikawa T, Ogino S, Kirchner T, Shivdasani RA. Differential WNT activity in colorectal cancer confers limited tumorigenic potential and is regulated by MAPK signaling. Cancer Res 2012; 72:1547-1556 | pdf | pubmed
Souglakos J, Philips J, Wang R, Marwah S, Silver M, Tzardi M, Silver J, Ogino S, Hooshmand S, Kwak E, Freed E, Meyerhardt JA, Saridaki Z, Georgoulias V, Finkelstein D, Fuchs CS, Kulke MH, Shivdasani RA. Prognostic and predictive value of common mutations for treatment response and survival in patients with metastatic colorectal cancer. Br J Cancer 2009; 101:465-472 | pdf | pubmed
Firestein R, Bass AJ, Kim S-Y, Dunn IF, Silver SJ, Guney I, Freed E, Ligon AH, Vena N, Ogino S, Chheda MG, Tamayo P, Finn S, Shrestha Y, Boehm JS, Jain S, Bojarski E, Mermel C, Barretina J, Chan JA, Baselga J, Tabernero J, Root DE, Fuchs CS, Loda M, Shivdasani RA, Meyerson M, Hahn WC. CDK8 is a colorectal cancer oncogene that regulates ß-catenin signaling. Nature 2008; 455:547-551 | pdf | pubmed
Kulke MH, Freed E, Chiang D, Philips J, Zahrieh D, Glickman JN, Shivdasani RA. High-resolution analysis of genetic alterations in small bowel carcinoid tumors reveals areas of recurrent amplification and loss. Genes Chromosomes Cancer 2008; 47:591-603 | pubmed
Lepourcelet M, Chen YP, Frances DS, Wang H, Crews P, Petersen F, Bruseo C, Wood AW, Shivdasani RA. Small molecule antagonists of the oncogenic beta-cetenin/Tcf protein complex. Cancer Cell 2004; 5:91-102 | pdf | pubmed
Livingston DM, Shivdasani RA. Toward mechanism-based cancer care. JAMA 2001; 285:588-593 [Review] | pubmed
Poy F, Lepourcelet M, Shivdasani RA, Eck MJ. Structure of a human Tcf4/b-catenin complex. Nature Struct Biol 2001; 8:1053-1057 | pdf | pubmed
Cejas P, Drier Y, Dreijerink KMA, Brosens LAA, Deshpande V, Epstein CB, Conemans EB, Morsink FHM, Graham MK, Valk GD, Vriens MR, Castillo CF, Ferrone CR, Adar T, Bowden M, Whitton HJ, Da Silva A, Font-Tello A, Long HW, Gaskell E, Shoresh N, Heaphy CM, Sicinska E, Kulke MH, Chung DC, Bernstein BE, Shivdasani RA. Enhancer signatures stratify and predict outcomes of non-functional pancreatic neuroendocrine tumors. Nat Med 2019; 25:1260-1265 | pdf | pubmed
Witwicki RM, Ekram MB, Qiu X, Janiszewska M, Shu S, Kwon M, Trinh A, Frias E, Ramadan N, Hoffman G, Yu K, Xie Y, McAllister G, McDonald R, Golji J, Schlabach M, deWeck A, Keen N, Chan HM, Ruddy D, Rejtar T, Sovath S, Silver S, Sellers WR, Jagani Z, Hogarty MD, Roberts CMW, Brown M, Stegmaier K, Long H, Shivdasani RA, Pellman D, Polyak K. TRPS1 is a lineage-specific transcriptional dependency in breast cancer. Cell Rep 2018; 25:1255-1267.e5 | pdf | pubmed
Schumacher SE, Shim BY, Corso G, Ryu MH, Kang YK, Roviello F, Saksena G, Peng S, Shivdasani RA, Bass AJ, Beroukhim R. Somatic copy number alterations in gastric adenocarcinomas among Asian and Western patients. PLoS One. 2017;12(4):e0176045 | pdf | pubmed
Cejas P, Cavazza A, Yandava CN, Moreno V, Horst D, Moreno-Rubio J, Burgos E, Mendiola M, Taing L, Goel A, Feliu J, Shivdasani RA. Transcriptional regulator CNOT3 defines an aggressive colorectal cancer subtype. Cancer Res 2017; 77:766-779 | pdf | pubmed
Cejas P, Li L, O'Neill NK, Duarte M, Rao P, Bowden M, Zhou CW, Mendiola M, Burgos E, Feliu J, Moreno-Rubio J, Guadalajara H, Moreno V, García-Olmo D, Bellmunt J, Mullane S, Hirsch M, Sweeney CJ, Richardson A, Liu XS, Brown M, Shivdasani RA, Long HW. Chromatin immunoprecipitation from fixed clinical tissues reveals tumor-specific enhancer profiles. Nat Med 2016; 22:685-691 | pdf | pubmed
Pomerantz MM, Li F, Takeda DY, Lenci R, Chonkar A, Chabot M, Cejas P, Vazquez F, Cook J, Shivdasani RA, Bowden M, Lis R, Hahn WC1,4, Kantoff PW1, Brown M, Loda M, Long HW, Freedman ML. The androgen receptor cistrome is extensively reprogrammed in human prostate tumorgenesis. Nat Genet 2015; 47:1346-1351 | pdf | pubmed
Sulahian R, Casey F, Shen J, Qian ZR, Shin H, Ogino S, Weir BA, Vazquez F, Liu XS, Hahn WC, Bass AJ, Chan V, Shivdasani RA. An integrative analysis reveals functional targets of GATA6 transcriptional regulation in gastric cancer. Oncogene 2014; 33:5637-5648 | pdf | pubmed
Francis J, Kiezun A, Ramos AH, Serra S, Pedamallu CS, Qian ZR, Banck MS, Kanwar R, Kulkarni AA, Karpathikis A, Manzo V, Contractor T, Philips J, Nickerson R, Pho N, Hooshmand SM, Brais LK, Lawrence M, Pugh T, McKenna A, Sivachenko A, Cibulskis K, Carter SL, Ojesina AI, Freeman S, Jones RT, Voet D, Saksena G, Auclair D, Onofrio R, Shefler E, Sougnez C, Grimsby J, Green L, Lennon N, Meyer T, Caplin M, Chung DC, Beutler AS, Ogino S, Thirlwell C, Shivdasani RA, Asa SL, Harris CR, Getz G, Kulke MH, Meyerson M. Somatic mutation of CDKN1B in small intestine neuroendocrine tumors. Nat Genet 2013; 45:1483-1486 | pdf | pubmed
Horst D, Chen J, Morikawa T, Ogino S, Kirchner T, Shivdasani RA. Differential WNT activity in colorectal cancer confers limited tumorigenic potential and is regulated by MAPK signaling. Cancer Res 2012; 72:1547-1556 | pdf | pubmed
Souglakos J, Philips J, Wang R, Marwah S, Silver M, Tzardi M, Silver J, Ogino S, Hooshmand S, Kwak E, Freed E, Meyerhardt JA, Saridaki Z, Georgoulias V, Finkelstein D, Fuchs CS, Kulke MH, Shivdasani RA. Prognostic and predictive value of common mutations for treatment response and survival in patients with metastatic colorectal cancer. Br J Cancer 2009; 101:465-472 | pdf | pubmed
Firestein R, Bass AJ, Kim S-Y, Dunn IF, Silver SJ, Guney I, Freed E, Ligon AH, Vena N, Ogino S, Chheda MG, Tamayo P, Finn S, Shrestha Y, Boehm JS, Jain S, Bojarski E, Mermel C, Barretina J, Chan JA, Baselga J, Tabernero J, Root DE, Fuchs CS, Loda M, Shivdasani RA, Meyerson M, Hahn WC. CDK8 is a colorectal cancer oncogene that regulates ß-catenin signaling. Nature 2008; 455:547-551 | pdf | pubmed
Kulke MH, Freed E, Chiang D, Philips J, Zahrieh D, Glickman JN, Shivdasani RA. High-resolution analysis of genetic alterations in small bowel carcinoid tumors reveals areas of recurrent amplification and loss. Genes Chromosomes Cancer 2008; 47:591-603 | pubmed
Lepourcelet M, Chen YP, Frances DS, Wang H, Crews P, Petersen F, Bruseo C, Wood AW, Shivdasani RA. Small molecule antagonists of the oncogenic beta-cetenin/Tcf protein complex. Cancer Cell 2004; 5:91-102 | pdf | pubmed
Livingston DM, Shivdasani RA. Toward mechanism-based cancer care. JAMA 2001; 285:588-593 [Review] | pubmed
Poy F, Lepourcelet M, Shivdasani RA, Eck MJ. Structure of a human Tcf4/b-catenin complex. Nature Struct Biol 2001; 8:1053-1057 | pdf | pubmed
Megakaryocytes and Platelets
Zang C, Luyten A, Chen J, Liu XS, Shivdasani RA. NF-E2, FLI1 and RUNX1 collaborate at areas of dynamic chromatin to activate transcription in mature mouse megakaryocytes. Sci Rep 2016;6:30255 | pdf | pubmed
Echtler K, Stark K, Lorenz M, Kerstan S, Walch A, Jennen L, Rudelius M, Seidl S, Kremmer E, Emambokus NR, von Bruehl M-L, Frampton J, Isermann B, Genzel-Boroviczény O, Schreiber C, Mehilli J, Kastrati A, Schwaiger M, Shivdasani RA, Massberg S. Platelets contribute to postnatal occlusion of the ductus arteriosus. Nature Medicine 2010; 16:75-82 | pdf | pubmed
Kunert S, Meyer I, Fleischhauer S, Wannack M, Fiedler J, Shivdasani RA, Schulze H. The microtubule modulator RanBP10 plays a critical role in regulation of platelet discoid shape and degranulation. Blood 2009; 114:5532-5540 | pdf | pubmed
Schulze H, Dose M, Korpal M, Meyer I, Italiano JE Jr, Shivdasani RA. RanBP10 is a cytoplasmic guanine nucleotide-exchange factor that modulates non-centrosomal microtubules. J Biol Chem 2008; 283:14109-14119 | pdf | pubmed
Patel-Hett S, Richardson JL, Schulze H, Drabek K, Isaac NA, Hoffmeister K, Shivdasani RA, Bulinski JC, Galjart N, Hartwig JH, Italiano JE Jr. Visualization of microtubule growth in living platelets reveals a dynamic marginal band with multiple microtubules. Blood 2008; 111:4605-4616 | pdf | pubmed
Junt T, Schulze H, Chen Z, Massberg S, Goerge T, Krueger A, Richardson J, Wagner DD, Graf T, Italiano JE, Shivdasani RA, von Andrian UH. Dynamic visualization of thrombopoiesis within bone marrow. Science 2007; 317:1767-1770 | pubmed
Chen Z, Naveiras O, Balduini A, Mammoto A, Conti MA, Adelstein RS, Ingber D, Daley GQ, Shivdasani RA. The May-Hegglin anomaly gene Myh9 is a negative regulator of platelet biogenesis modulated by the Rho-ROCK pathway. Blood 2007; 110:171-179 | pdf | pubmed
Chen Z, Hu M, Shivdasani RA. Expression analysis of primary mouse megakaryocyte differentiation and its application to identify stage-specific molecular markers and a novel transcriptional target of NF-E2. Blood 2007; 109:1451-1459 | pdf | pubmed
Schulze H, Korpal M, Hurov J, Kim S-W, Zhang J, Cantley LC, Graf T, Shivdasani RA. Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis. Blood 2006; 107:3868-3875 | pdf | pubmed
Richardson J, Shivdasani RA, Boers C, Hartwig JH, Italiano JE Jr. Mechanisms of organelle transport and capture along proplatelets during platelet production. Blood 2005; 106:4066-4075 | pdf | pubmed
Patel SR, Richardson J, Schulze H, Kahle E, Galjart N, Drabek K, Shivdasani RA, Hartwig JH, Italiano JE Jr. Differential roles of microtubule assembly and sliding in proplatelet formation by megakaryocytes. Blood 2005; 106:4076-4085 | pdf | pubmed
Schulze H, Korpal M, Bergmeier W, Italiano JE, Wahl SM, Shivdasani RA. Interactions between the megakaryocyte/platelet-specific beta-1 tubulin and the secretory leukocyte protease inhibitor SLPI suggest a role for regulated proteolysis in platelet functions. Blood, 2004; 104: 3949-3957 | pdf | pubmed
Tiwari S, Italiano JE, Barral DC, Mules EH, Novak EK, Swank RT, Seabra MC, Shivdasani RA. A role for Rab27b in NF-E2-dependent pathways of platelet formation. Blood, 2003; 102:3970-3979 | pdf | pubmed
Italiano JE, Bergmeier W, Tiwari S, Falet H, Hartwig JH, Hoffmeister K, Andre P, Wagner DD, Shivdasani RA. Mechanisms and implications of platelet discoid shape. Blood 2003; 101:4789-4796 | pdf | pubmed
Italiano JE, Shivdasani RA. Megakaryocytes and beyond: the birth of platelets. J Thromb Haemost, 2003; 1:1174-1181 | pdf | pubmed
Shivdasani RA. Molecular and transcriptional regulation of megakaryocyte differentiation. Stem Cells 2001; 19:397-407 [Review] | pdf | pubmed
Schwer HD, Lecine P, Tiwari S, Italiano JE, Hartwig JH, Shivdasani RA. A lineage-restricted and divergent beta-tubulin isoform is essential for the biogenesis, structure and function of blood platelets. Curr Biol 2001; 11:579-586 | pdf | pubmed
Lecine P, Italiano JE, Kim SW, Villeval J-L, Shivdasani RA. Hematopoietic-specific beta1 tubulin participates in a pathway of platelet biogenesis dependent on the transcription factor NF-E2. Blood 2000; 96:1366-1373 | pdf | pubmed
Vyas P, Ault K, Jackson CW, Orkin SH, Shivdasani RA. Consequences of GATA-1 deficiency in megakaryocytes and platelets. Blood 1999; 93:2867-2875 | pdf | pubmed
Levin J, Peng J-P, Baker GR, Villeval J-L, Lecine P, Burstein SA, Shivdasani RA. Pathophysiology of thrombocytopenia and anemia in mice lacking transcription factor NF-E2. Blood 1999; 94: 3037-3047 | pdf | pubmed
Italiano JE, Lecine P, Shivdasani RA, Hartwig JH. Blood platelets are assembled principally at the ends of proplatelet processes produced by differentiated megakaryocytes. J Cell Biol 1999; 147:1299-1312 | pdf | pubmed
de Sauvage FJ, Villeval J-L, Shivdasani RA. Regulation of megakaryocytopoiesis and platelet production: Lessons from animal models. J Lab Clin Med 1998; 131:496-501 [Review] | pubmed
Lecine P, Blank V, Shivdasani RA. Characterization of the hematopoietic transcription factor NF-E2 in primary murine megakaryocytes. J Biol Chem 1998; 273:7572-7578 | pdf | pubmed
Lecine P, Villeval J-L, Vyas P, Swencki B, Xu Y, Shivdasani RA. Mice lacking transcription factor NF-E2 provide in vivo validation of the proplatelet model of thrombocytopoiesis and show a platelet production defect that is intrinsic to megakaryocytes. Blood 1998; 92:1609-1616 | pdf | pubmed
Shivdasani RA, Fujiwara Y, McDevitt MA, Orkin SH. A lineage-selective knockout establishes the critical role of transcription factor GATA-1 in megakaryocyte growth and platelet development. EMBO J 1997; 16:3965-3973 | pdf | pubmed
Shivdasani RA, Fielder P, Keller G-A, Orkin SH, de Sauvage FJ. Regulation of the serum concentra-tion of thrombopoietin in thrombocytopenic NF-E2 knockout mice. Blood 1997; 90:1821-1827 | pdf | pubmed
Shivdasani RA, Rosenblatt MF, Zucker-Franklin D, Jackson CW, Hunt P, Saris CJM, Orkin SH. Transcription factor NF-E2 is required for platelet formation independent of the actions of thrombopoietin/MGDF in megakaryocyte development. Cell 1995; 81:695-704 | pdf | pubmed
Zang C, Luyten A, Chen J, Liu XS, Shivdasani RA. NF-E2, FLI1 and RUNX1 collaborate at areas of dynamic chromatin to activate transcription in mature mouse megakaryocytes. Sci Rep 2016;6:30255 | pdf | pubmed
Echtler K, Stark K, Lorenz M, Kerstan S, Walch A, Jennen L, Rudelius M, Seidl S, Kremmer E, Emambokus NR, von Bruehl M-L, Frampton J, Isermann B, Genzel-Boroviczény O, Schreiber C, Mehilli J, Kastrati A, Schwaiger M, Shivdasani RA, Massberg S. Platelets contribute to postnatal occlusion of the ductus arteriosus. Nature Medicine 2010; 16:75-82 | pdf | pubmed
Kunert S, Meyer I, Fleischhauer S, Wannack M, Fiedler J, Shivdasani RA, Schulze H. The microtubule modulator RanBP10 plays a critical role in regulation of platelet discoid shape and degranulation. Blood 2009; 114:5532-5540 | pdf | pubmed
Schulze H, Dose M, Korpal M, Meyer I, Italiano JE Jr, Shivdasani RA. RanBP10 is a cytoplasmic guanine nucleotide-exchange factor that modulates non-centrosomal microtubules. J Biol Chem 2008; 283:14109-14119 | pdf | pubmed
Patel-Hett S, Richardson JL, Schulze H, Drabek K, Isaac NA, Hoffmeister K, Shivdasani RA, Bulinski JC, Galjart N, Hartwig JH, Italiano JE Jr. Visualization of microtubule growth in living platelets reveals a dynamic marginal band with multiple microtubules. Blood 2008; 111:4605-4616 | pdf | pubmed
Junt T, Schulze H, Chen Z, Massberg S, Goerge T, Krueger A, Richardson J, Wagner DD, Graf T, Italiano JE, Shivdasani RA, von Andrian UH. Dynamic visualization of thrombopoiesis within bone marrow. Science 2007; 317:1767-1770 | pubmed
Chen Z, Naveiras O, Balduini A, Mammoto A, Conti MA, Adelstein RS, Ingber D, Daley GQ, Shivdasani RA. The May-Hegglin anomaly gene Myh9 is a negative regulator of platelet biogenesis modulated by the Rho-ROCK pathway. Blood 2007; 110:171-179 | pdf | pubmed
Chen Z, Hu M, Shivdasani RA. Expression analysis of primary mouse megakaryocyte differentiation and its application to identify stage-specific molecular markers and a novel transcriptional target of NF-E2. Blood 2007; 109:1451-1459 | pdf | pubmed
Schulze H, Korpal M, Hurov J, Kim S-W, Zhang J, Cantley LC, Graf T, Shivdasani RA. Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis. Blood 2006; 107:3868-3875 | pdf | pubmed
Richardson J, Shivdasani RA, Boers C, Hartwig JH, Italiano JE Jr. Mechanisms of organelle transport and capture along proplatelets during platelet production. Blood 2005; 106:4066-4075 | pdf | pubmed
Patel SR, Richardson J, Schulze H, Kahle E, Galjart N, Drabek K, Shivdasani RA, Hartwig JH, Italiano JE Jr. Differential roles of microtubule assembly and sliding in proplatelet formation by megakaryocytes. Blood 2005; 106:4076-4085 | pdf | pubmed
Schulze H, Korpal M, Bergmeier W, Italiano JE, Wahl SM, Shivdasani RA. Interactions between the megakaryocyte/platelet-specific beta-1 tubulin and the secretory leukocyte protease inhibitor SLPI suggest a role for regulated proteolysis in platelet functions. Blood, 2004; 104: 3949-3957 | pdf | pubmed
Tiwari S, Italiano JE, Barral DC, Mules EH, Novak EK, Swank RT, Seabra MC, Shivdasani RA. A role for Rab27b in NF-E2-dependent pathways of platelet formation. Blood, 2003; 102:3970-3979 | pdf | pubmed
Italiano JE, Bergmeier W, Tiwari S, Falet H, Hartwig JH, Hoffmeister K, Andre P, Wagner DD, Shivdasani RA. Mechanisms and implications of platelet discoid shape. Blood 2003; 101:4789-4796 | pdf | pubmed
Italiano JE, Shivdasani RA. Megakaryocytes and beyond: the birth of platelets. J Thromb Haemost, 2003; 1:1174-1181 | pdf | pubmed
Shivdasani RA. Molecular and transcriptional regulation of megakaryocyte differentiation. Stem Cells 2001; 19:397-407 [Review] | pdf | pubmed
Schwer HD, Lecine P, Tiwari S, Italiano JE, Hartwig JH, Shivdasani RA. A lineage-restricted and divergent beta-tubulin isoform is essential for the biogenesis, structure and function of blood platelets. Curr Biol 2001; 11:579-586 | pdf | pubmed
Lecine P, Italiano JE, Kim SW, Villeval J-L, Shivdasani RA. Hematopoietic-specific beta1 tubulin participates in a pathway of platelet biogenesis dependent on the transcription factor NF-E2. Blood 2000; 96:1366-1373 | pdf | pubmed
Vyas P, Ault K, Jackson CW, Orkin SH, Shivdasani RA. Consequences of GATA-1 deficiency in megakaryocytes and platelets. Blood 1999; 93:2867-2875 | pdf | pubmed
Levin J, Peng J-P, Baker GR, Villeval J-L, Lecine P, Burstein SA, Shivdasani RA. Pathophysiology of thrombocytopenia and anemia in mice lacking transcription factor NF-E2. Blood 1999; 94: 3037-3047 | pdf | pubmed
Italiano JE, Lecine P, Shivdasani RA, Hartwig JH. Blood platelets are assembled principally at the ends of proplatelet processes produced by differentiated megakaryocytes. J Cell Biol 1999; 147:1299-1312 | pdf | pubmed
de Sauvage FJ, Villeval J-L, Shivdasani RA. Regulation of megakaryocytopoiesis and platelet production: Lessons from animal models. J Lab Clin Med 1998; 131:496-501 [Review] | pubmed
Lecine P, Blank V, Shivdasani RA. Characterization of the hematopoietic transcription factor NF-E2 in primary murine megakaryocytes. J Biol Chem 1998; 273:7572-7578 | pdf | pubmed
Lecine P, Villeval J-L, Vyas P, Swencki B, Xu Y, Shivdasani RA. Mice lacking transcription factor NF-E2 provide in vivo validation of the proplatelet model of thrombocytopoiesis and show a platelet production defect that is intrinsic to megakaryocytes. Blood 1998; 92:1609-1616 | pdf | pubmed
Shivdasani RA, Fujiwara Y, McDevitt MA, Orkin SH. A lineage-selective knockout establishes the critical role of transcription factor GATA-1 in megakaryocyte growth and platelet development. EMBO J 1997; 16:3965-3973 | pdf | pubmed
Shivdasani RA, Fielder P, Keller G-A, Orkin SH, de Sauvage FJ. Regulation of the serum concentra-tion of thrombopoietin in thrombocytopenic NF-E2 knockout mice. Blood 1997; 90:1821-1827 | pdf | pubmed
Shivdasani RA, Rosenblatt MF, Zucker-Franklin D, Jackson CW, Hunt P, Saris CJM, Orkin SH. Transcription factor NF-E2 is required for platelet formation independent of the actions of thrombopoietin/MGDF in megakaryocyte development. Cell 1995; 81:695-704 | pdf | pubmed
Developmental Hematopoiesis
Luyten A, Zang C, Liu XS, Shivdasani RA. Active enhancers are delineated de novo during hematopoiesis, with limited lineage fidelity among specified primary blood cells. Genes Dev 2014; 28:1827-1839 | pdf | pubmed
Schulze H, Korpal M, Hurov J, Kim S-W, Zhang J, Cantley LC, Graf T, Shivdasani RA. Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis. Blood 2006; 107:3868-3875 | pdf | pubmed
Kacena MA, Shivdasani RA, Wilson K, Xi Y, Troiano N, Nazarian A, Gundberg CM, Bouxsein ML, Lorenzo JA, Horowitz MC. Megakaryocyte-osteoblast interaction revealed in mice deficient in transcription factors GATA-1 and NF-E2. J Bone Miner Res 2004; 19:652-660 | pdf | pubmed
McDevitt MA, Fujiwara Y, Shivdasani RA, Orkin SH. An upstream, DNaseI hypersensitive region of the hematopoietic-expressed transcription factor GATA-1 gene confers developmental specificity in transgenic mice. Proc Natl Acad Sci USA 1997; 94:7976-7981 | pdf | pubmed
Shivdasani RA, Orkin SH. The transcriptional control of hematopoiesis. Blood 1996; 87:4025-4039 [Review] | pdf | pubmed
Peters LL, Shivdasani RA, Liu SC, Hanspal M, John KM, Gonzalez JM, Brugnara C, Gwynn B, Mohandas N, Alper SL, Orkin SH, Lux SE. Anion exchanger 1 (Band 3) is required to prevent erythrocyte membrane surface loss but not to form the membrane skeleton. Cell 1996; 86:917-927 | pdf | pubmed
Shivdasani RA, Mayer EL, Orkin SH. Absence of blood formation in mice lacking the T-cell leukaemia oncoprotein Tal-1/SCL. Nature 1995; 373:432-434 | pubmed
Shivdasani RA, Orkin SH. Erythropoiesis and globin gene expression in mice lacking the transcription factor NF-E2. Proc Natl Acad Sci USA 1995; 92:8690-8694 | pdf | pubmed
Luyten A, Zang C, Liu XS, Shivdasani RA. Active enhancers are delineated de novo during hematopoiesis, with limited lineage fidelity among specified primary blood cells. Genes Dev 2014; 28:1827-1839 | pdf | pubmed
Schulze H, Korpal M, Hurov J, Kim S-W, Zhang J, Cantley LC, Graf T, Shivdasani RA. Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis. Blood 2006; 107:3868-3875 | pdf | pubmed
Kacena MA, Shivdasani RA, Wilson K, Xi Y, Troiano N, Nazarian A, Gundberg CM, Bouxsein ML, Lorenzo JA, Horowitz MC. Megakaryocyte-osteoblast interaction revealed in mice deficient in transcription factors GATA-1 and NF-E2. J Bone Miner Res 2004; 19:652-660 | pdf | pubmed
McDevitt MA, Fujiwara Y, Shivdasani RA, Orkin SH. An upstream, DNaseI hypersensitive region of the hematopoietic-expressed transcription factor GATA-1 gene confers developmental specificity in transgenic mice. Proc Natl Acad Sci USA 1997; 94:7976-7981 | pdf | pubmed
Shivdasani RA, Orkin SH. The transcriptional control of hematopoiesis. Blood 1996; 87:4025-4039 [Review] | pdf | pubmed
Peters LL, Shivdasani RA, Liu SC, Hanspal M, John KM, Gonzalez JM, Brugnara C, Gwynn B, Mohandas N, Alper SL, Orkin SH, Lux SE. Anion exchanger 1 (Band 3) is required to prevent erythrocyte membrane surface loss but not to form the membrane skeleton. Cell 1996; 86:917-927 | pdf | pubmed
Shivdasani RA, Mayer EL, Orkin SH. Absence of blood formation in mice lacking the T-cell leukaemia oncoprotein Tal-1/SCL. Nature 1995; 373:432-434 | pubmed
Shivdasani RA, Orkin SH. Erythropoiesis and globin gene expression in mice lacking the transcription factor NF-E2. Proc Natl Acad Sci USA 1995; 92:8690-8694 | pdf | pubmed